Soheila Sayad
1 
, Mahshid Panahi
2, Reza Nekouian
3,4* , Saed Sayad
51 Department of Breast Surgery, Firoozgar Clinical Research Development Center, Iran University of Medical Sciences, Tehran, Iran
2 Department of Pathology, Firoozgar Clinical Research Development Center, Iran University of Medical Sciences, Tehran, Iran
3 Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
4 Department of Medical Biotechnology, Iran University of Medical Sciences, Tehran, Iran
5 Department of Computer Science, Rutgers University, New Jersey, USA
Abstract
Sustained angiogenesis is a hallmark of solid tumors. Nowadays, a lot of studies are conducted to investigate angiogenesis and angiogenic pathways. There are many anticancer agents targeting these pathways. However, there is not a clinical method to detect or quantify angiogenesis. In ImageJ software, color threshold of angiogenesis can be defined. However, this method is not specific for hematoxylin and eosin stained slides.