Sustained angiogenesis is a hallmark of solid tumors. Nowadays, a lot of studies are conducted to investigate angiogenesis and angiogenic pathways. There are many anticancer agents targeting these pathways. However, there is not a clinical method to detect or quantify angiogenesis. In ImageJ software, color threshold of angiogenesis can be defined. However, this method is not specific for hematoxylin and eosin stained slides.