Society of Diabetic Nephropathy Prevention Journal of Preventive Epidemiology 2476-3934 4 2 2019 07 01 Is software method helpful for detection of angiogenesis in breast tissue slides? e16 e16 EN Soheila Sayad https://orcid.org/0000-0003-3904-7394 Mahshid Panahi Reza Nekouian https://orcid.org/0000-0002-8348-8368 Saed Sayad LETTER 2020 01 18 2020 02 08 Sustained angiogenesis is a hallmark of solid tumors. Nowadays, a lot of studies are conducted to investigate angiogenesis and angiogenic pathways. There are many anticancer agents targeting these pathways. However, there is not a clinical method to detect or quantify angiogenesis. In ImageJ software, color threshold of angiogenesis can be defined. However, this method is not specific for hematoxylin and eosin stained slides.